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Saturday, December 18, 2004

Microbiology Video Library

This is a microbiology video library from the University of Leicester. There are over 300 videos of microbiology topics listed in alphabetical order. The topics are listed in alphabetical order, or you can click on the letter if you know the name of a topic Microbiology and Immunology Online Index

Saturday, November 20, 2004

Mitochondrial DNA Knowledge

This page explains in detail an introduction to understanding mitochondrial DNA. It also includes laboratory methods such as PCR, D-probe preparation, and the Southern Blot assay. A results section shows results of a human placenta sample treated with RNAse Agarose Gel stained with ethidium bromide.
See the entire page at this link>>> Genetics and Molecular Research- mitochondrial DNA from human placenta.

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Top-Pick website on Infectious Diseases

This site is an A to Z index of infectious diseases as written in the Material Safety Data Sheet of Infectious Diseases by the Public Health Agency. The descriptions include: Cross-references, Health Hazards, Communicability, Laboratory Hazards, Viability, and others.
It is an excellent site for reference information on pathogenic organisms.
MSDS of Infectious Diseases

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Monday, November 15, 2004

DNA Forensics

The DNA forensics link below includes the definition, background, and processes of DNA forensics. DNA profiled on this website is the case of : "The state of California vs. O.J. Simpson on the count of murder in the first degree of one person, and the count of murder in the second-degree of a second person".
Some knowledge of: genetic coding, mitochondrial DNA, polymerase chain reactions(PCR), blood typing and crossmatching, and laboratory techniques, is necessary for understanding this page.
DNA Forensics at work website
Note: The webpage is in Adobe Reader format.
Download Adobe

Reminder: This webpage, "Class Notes", is without pedjudice to religion, sex, race, or national origin. All information is purely instructional and is intended for Microbiology students. All of the information printed is of public record, reprinted with granted permission of instructors, or both.

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Wednesday, November 10, 2004

Suggested Reading

Here is a selection about Barbara Kingsolver's views on Genetic Engineering. The story entails Christianity, Darwanism, and other aspects of life on Earth and the effects of Biotechnology.
It's from her new book, "Small Wonders".
You can read the link here, or the hardcover is also available.
"A Fist in the Eye of God"

(Note: You can post comments and thoughts of the story in this thread..)

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Sunday, November 07, 2004

DNA Translation Animaton

Here is an easy to follow tutorial of protein synthesis and DNA translation from the University of Nebraska.

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Friday, October 29, 2004

Antiseptics and Antibiotics (Kirby-Bauer test)

Antibiotics are defined as a chemical product that can inhibit or destroy pathogenic microorganisms.
Antiseptics are defined as chemicals that are able to inhibit in vitro sepsis. They do not kill the sepsis-producing agent, but inhibit its growth. Antiseptics are nontoxic to allow application to the skin and mucous membranes, such as Listerine.
The mode of action for these chemicals is the active denaturing of proteins within pathogenic organisms, cytoplasmic membrane dissolution, and act as oxidizing agents.
Clinical laboratories can test the potency of antibiotics and antiseptics by using the Kirby-Bauer test.A second example on this page includes a table for interpreting results according to critical diameters. You can determine from this table, assuming the test was done properly, if the bacterial agent is resistant, intermediate, or susceptible to the antibiotic that is being questioned.
Mueller-Hinton agar is the usual agar of choice for this procedure.
This test should be monitored closely, especially in the professional laboratory. Time limits and culture inoculum sizes should be within requirements for proper results. (Collegiate labs use a less restrictive Kirby-Bauer method).

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Thursday, October 21, 2004

Streptomyces


Streptomyces can form unique spores even in the presence of numerous antibiotics. They are the most successful bacterium in the Order Actinomycetales. They are found worldwide in soil and are important in soil ecology. In fact, much of the earthly smell of soil are given off by the Strptomyces species. They are also found in the medical industry because they synthesize antibiotics. They can eat almost anything, including sugars, alcohols, amino acids, and organic acids.
Click here to watch a cool clip!
If you don't have the required Quick Time Player, you can download it here.
Work cited: University of Leicester- Department of Microbiology and Immunology